产品
编 号：F170805
分子式：C27H33IN2
分子量：512.47
分子式：C27H33IN2
分子量：512.47
产品类型
规格
价格
是否有货
50mg
120
In-stock
100mg
192
In-stock
结构图
CAS No: 14696-39-0
产品详情
生物活性：
Astrophloxine is a fluorescent imaging probe capable of targeting antiparallel dimers. Astrophloxine can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples of Alzheimer's disease (AD) mice.
体内研究：
Astrophloxine shows more fluorescence (A.U.) in APP/PS1 mice CSF rather in WT mice, and is able to indicate soluble Aβ oligomers in CSF and insoluble plaques in brain tissue.
体外研究：
Astrophloxine binds Aβ dimers with stronger fluorescence intensity (A.U.) than binding Aβ monomers.Protocol for CSF analyses:1.Prepare 1 μL of CSF samples with 1 μL of 10X protease inhibitors and diluted the mixture with 8 μL of binding buffer. Binding buffer (0.1 M sodium phosphate, 0.15 M sodium chloride, 10 mM EDTA; pH 7.2).2.Dilute Astrophloxine to 0.5 μM with binding buffer.3.Add individual samples and dye to the wells of a 96-well half-area black microplate with a sample to dye ratio of 1:3.Detect at λex=540 nm/λem=570 nm.
Astrophloxine is a fluorescent imaging probe capable of targeting antiparallel dimers. Astrophloxine can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples of Alzheimer's disease (AD) mice.
体内研究：
Astrophloxine shows more fluorescence (A.U.) in APP/PS1 mice CSF rather in WT mice, and is able to indicate soluble Aβ oligomers in CSF and insoluble plaques in brain tissue.
体外研究：
Astrophloxine binds Aβ dimers with stronger fluorescence intensity (A.U.) than binding Aβ monomers.Protocol for CSF analyses:1.Prepare 1 μL of CSF samples with 1 μL of 10X protease inhibitors and diluted the mixture with 8 μL of binding buffer. Binding buffer (0.1 M sodium phosphate, 0.15 M sodium chloride, 10 mM EDTA; pH 7.2).2.Dilute Astrophloxine to 0.5 μM with binding buffer.3.Add individual samples and dye to the wells of a 96-well half-area black microplate with a sample to dye ratio of 1:3.Detect at λex=540 nm/λem=570 nm.
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