产品
编 号：F270226
分子式：C38H37F3N8O11
分子量：838.74
分子式：C38H37F3N8O11
分子量：838.74
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规格
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结构图
CAS No: 210344-99-3
产品详情
生物活性：
Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Caspase-1 Fluorogenic Activity Assay:1. Incubate the cells according to your normal protocol.2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.
Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Caspase-1 Fluorogenic Activity Assay:1. Incubate the cells according to your normal protocol.2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.
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