产品
编 号：F346316
产品类型
规格
价格
是否有货
25mg
400
In-stock
50mg
600
In-stock
100mg
960
In-stock
500mg
2800
In-stock
结构图
CAS No: 39450-01-6
产品详情
生物活性：
Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K can be use for promoting methods of viral nucleic acid extraction, and detection.
体外研究：
Proteinase K enzyme can be used to break amid bond between MTX and BSA and also amidic bonds in BSA structure.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Proteinase K with superior protein-degrading property, can be used to detect the SARS-COV2 viral component directly from inoculated viral transport medium (VTM) without RNA extraction. Protocol of rRTPCR detection of SARS-CoV2:1.Separate VTM of each SARS-COV2 samples into three EP tubes (45 μL per tube);2.Add 2.5 μL (20 mg/mL concentrated) proteinase K (PK), making a final concentration of PK ~1 mg/ml VTM;3.Incubate at 25 ℃ for 5 min;4.Incubate at 60 ℃ for 15 min;5.Incubate at 98 ℃ for 10 min, and store samples at 4 ℃;6.Use 5 μL of extracted RNA as template to execute rRTPCR assay;7.Exert rRTPCR detection by BIORAD CFX96? Real-Time Thermocycler, with a positive standard of threshold cycle (Ct) values less than 40.Proteinase K improves the homogenization effects and positive rates of sputum samples, and increases nucleic acid concentration extracted from it.Protocol of nucleic acid extraction of influenza A virus (IAV):1.Separate specimen into three EP tubes (500 μL per tube);2.Add 500 μL 0.4 mg/mL Proteinase K buffer, vortex for 20 seconds;3.Incubate at 55 ℃ for 25 min, vortex 5 seconds every 5 min;4.Extract RNA by using the Automated Nucleic Acid Extraction System through a paramagnetic beads method (Zhijiang Biotechnology Co., Ltd., Shanghai, China) with 200 μL samples;5.Evaluate A260/A280 ratio of pure RNA products, with a standard ranged from 1.8 to 2.0.
Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K can be use for promoting methods of viral nucleic acid extraction, and detection.
体外研究：
Proteinase K enzyme can be used to break amid bond between MTX and BSA and also amidic bonds in BSA structure.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Proteinase K with superior protein-degrading property, can be used to detect the SARS-COV2 viral component directly from inoculated viral transport medium (VTM) without RNA extraction. Protocol of rRTPCR detection of SARS-CoV2:1.Separate VTM of each SARS-COV2 samples into three EP tubes (45 μL per tube);2.Add 2.5 μL (20 mg/mL concentrated) proteinase K (PK), making a final concentration of PK ~1 mg/ml VTM;3.Incubate at 25 ℃ for 5 min;4.Incubate at 60 ℃ for 15 min;5.Incubate at 98 ℃ for 10 min, and store samples at 4 ℃;6.Use 5 μL of extracted RNA as template to execute rRTPCR assay;7.Exert rRTPCR detection by BIORAD CFX96? Real-Time Thermocycler, with a positive standard of threshold cycle (Ct) values less than 40.Proteinase K improves the homogenization effects and positive rates of sputum samples, and increases nucleic acid concentration extracted from it.Protocol of nucleic acid extraction of influenza A virus (IAV):1.Separate specimen into three EP tubes (500 μL per tube);2.Add 500 μL 0.4 mg/mL Proteinase K buffer, vortex for 20 seconds;3.Incubate at 55 ℃ for 25 min, vortex 5 seconds every 5 min;4.Extract RNA by using the Automated Nucleic Acid Extraction System through a paramagnetic beads method (Zhijiang Biotechnology Co., Ltd., Shanghai, China) with 200 μL samples;5.Evaluate A260/A280 ratio of pure RNA products, with a standard ranged from 1.8 to 2.0.
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