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编 号：F579845
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CAS No: 9002-04-4
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Thrombin (MW 37kDa) is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
体外研究：
p62 is cleaved from the agarose beads as well as the GST tag by addition of Thrombin (1 Unit/100 μg protein) at room temperature for 20 h.Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
Thrombin (MW 37kDa) is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
体外研究：
p62 is cleaved from the agarose beads as well as the GST tag by addition of Thrombin (1 Unit/100 μg protein) at room temperature for 20 h.Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
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