生物活性：
Tumour Necrosis Factor alpha (TNF alpha) is a potent pro-inflammatory cytokine. TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death. TNF alpha stimulates NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis. Anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse. TNF alpha as a proneurogenic factor activates the SAPK/JNK Pathway and can facilitate neuronal replacement and brain repair in response to brain injury[4]. TNF alpha/TNFSF2 protein, Human (His) is a recombinant protein with a C-Terminal His label, It consists of 157 amino acids (V77-L233) and is produced in CHO cells.

体内活性：
TNF alpha (重组人源蛋白) (100 µg; 后背植入锇泵; 每日注入，共 7 天) 在 SCID-HuRAg 小鼠中，显着增加炎症细胞的数量，并且滑膜炎症的程度显着加剧。

体外活性：
TNF alpha (重组人源蛋白) (0, 10, 15, 20, 30, 50 ng/mL; 24, 48 h) 在 30 ng/mL 浓度，48 小时后降低 KB-C1 细胞活力，减少 pro-caspase-3，裂解的 caspase -3 在 KB-C1 和 KB-3-1 细胞中的表达. TNF alpha (重组人源蛋白) (10, 15 ng/mL; 24 h) 显着增加 KB-3-1 细胞中 Pgp (ABCB1) 的 mRNA 水平，促进总 Pgp 蛋白水平的表达降低，轻微降低 KB-C1 细胞表面的的 Pgp 蛋白水平，并可能通过 ERK 激活刺激 IHF 细胞增殖。