产品
编 号：F086656
分子式：C21H20BrN3
分子量：394.31
分子式：C21H20BrN3
分子量：394.31
产品类型
规格
价格
是否有货
10mM*1mL in DMSO
264
In-stock
100mg
240
In-stock
250mg
400
In-stock
结构图
CAS No: 1239-45-8
产品详情
生物活性：
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).?The preparation of agarose gel:?1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.?2. Mix the agarose powder with 1X TAE/TBE.?3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).?4. Make the solution cool down before solidification.?5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel).?6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.?CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
体外研究：
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).?The preparation of agarose gel:?1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.?2. Mix the agarose powder with 1X TAE/TBE.?3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).?4. Make the solution cool down before solidification.?5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel).?6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.?CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
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